Application

Research CategoryNeuroscience

Research Sub CategoryNeurochemistry & Neurotrophins

Immunohistochemistry at 1:500-1:5,000 (see suggested protocol).

Immunoblotting at 1:500-1:5,000.

ELISA at 1:500-1:5,000.

Inhibition of biological activity in vitro at 1:10-1:50.
BIOLOGICAL ACTIVITY: Neutralizes NGF, but not other neurotrophins.

Use neat for in vivo animal studies (5-10 µL/g body weight).

Optimal working dilutions must be determined by end user.

Detect Nerve Growth Factor-β using this Anti-Nerve Growth Factor-β Antibody validated for use in ELISA, WB, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Mouse nerve growth factor-beta purified from salivary glands.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Whole sheep antisera. Lyophilized. Reconstitute with 250 µL of sterile distilled water.

Specificity

Nerve growth factor-beta (NGF). Less than 1% cross-reactivity against recombinant human NT4 and BDNF and approximately 2% for NT3 by ELISA.

Storage and Stability

Maintain at -20°C to -70°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.